In Vitro Determination of Chlorophyll a and Pheophytin a in Marine and Freshwater Algae by Fluorescence




This method provides a procedure for low level determination of chlorophyll a (chl a) and its magnesiumfree derivative, pheophytin a (pheo a), in marine and freshwater phytoplankton using fluorescence detection.(1,2) Phaeophorbides present in the sample are determined collectively as pheophytin a. For users primarily interested in chl a there is currently available a set of very narrow bandpass excitation and emission filters (Turner Designs, Sunnyvale, CA) that nearly eliminates the spectral interference caused by the presence of pheo a and chlorophyll b.


National Exposure Research Laboratory Office of Research and Development






- N/A


- Inglês


- Nenhuma restrição


- Biodiversidade Marinha


- Relatórios, Estudos, Avaliações de Biodiversidade, Linhas de Base


- Uso Sustentável da Biodiversidade


- Nenhuma / Não aplicável



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